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| Ribeiro,L.A.; Mariani,P.D.S.C.; Azevedo,J.L.; Rech,E.L.; Schmidt,G.S.; Coutinho,L.L.. |
| Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions obtained for high embryo survival and high transformation efficiency were achieved with 800 psi helium gas pressure, 500 mmHg vacuum, gold particles, an 8 cm DNA-coated microparticle flying distance to the embryo and embryo placement 0.5 cm from the center of the particle dispersion cone. Under these conditions, transformation efficiency was 100%, survival rate 25% and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biolistic process; Chicken embryo; Gene transfer; SS-galactosidase; Green fluorescent protein; GFP. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000900003 |
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| RIOS,MARIANA; VENEGAS,ALEJANDRO; CROXATTO,HORACIO B. |
| Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (<A HREF="#rios97">Ríos et al., 1997</A>). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal... |
| Tipo: Journal article |
Palavras-chave: SS-galactosidase; Gene transfer; Oviduct. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007 |
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